› 6nx9

X-ray diffraction
1.97Å resolution

ECAII(D90T,K162T) MUTANT IN COMPLEX WITH CITRATE AT PH 7

Released:
DOI: 10.2210/pdb6nx9/pdb
Model geometry
Fit model/data
PDB entry 6nx9 coloured by chain, front view.
PDB entry 6nx9 coloured by chain, side view.
PDB entry 6nx9 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Opportunistic complexes of E. coli L-asparaginases with citrate anions.
Lubkowski J, Chan W, Wlodawer A
OpenAccess logo Sci Rep 9 11070 (2019)
PMID: 31363102

Function and Biology Details

Reaction catalysed: 
L-asparagine + H(2)O = L-aspartate + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133590 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-asparaginase 2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 333 amino acids
Theoretical weight: 35.54 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00805 (Residues: 23-348; Coverage: 100%)
Gene names: JW2924, ansB, b2957
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand ACY 4 x ACY
Ligand IMD 1 x IMD
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2
Unit cell:
a: 152.009Å b: 62.539Å c: 143.239Å
α: 90° β: 118.19° γ: 90°
R-values:
R R work R free
0.162 0.16 0.226
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Structural and biophysical aspects of l-asparaginases: a growing family with amazing diversity.
Loch et al. (2021)
4 other articles

1 mention without citation

Opportunistic complexes of E. coli L-asparaginases with citrate anions.
Lubkowski et al. (2019)