› 6nyc

X-ray diffraction
1.89Å resolution

Munc13-1 C2B-domain, calcium free

Released:
DOI: 10.2210/pdb6nyc/pdb
PDB entry 6nyc coloured by chain, front view.
PDB entry 6nyc coloured by chain, side view.
PDB entry 6nyc coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Munc13 C2B domain is an activity-dependent Ca2+ regulator of synaptic exocytosis.
Shin OH, Lu J, Rhee JS, Tomchick DR, Pang ZP, Wojcik SM, Camacho-Perez M, Brose N, Machius M, Rizo J, Rosenmund C, Südhof TC
Nat Struct Mol Biol 17 280-8 (2010)
PMID: 20154707

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178967 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein unc-13 homolog A Chain: A
Molecule details ›
Chain: A
Length: 148 amino acids
Theoretical weight: 16.94 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q62768 (Residues: 675-820; Coverage: 8%)
Gene names: Unc13a, Unc13h1
Sequence domains: C2 domain
Structure domains: C2 domain

Ligands and Environments

2 bound ligands:
Ligand CL 2 x CL
Ligand B3P 1 x B3P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: C2221
Unit cell:
a: 42.572Å b: 101.141Å c: 68.038Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.209 0.248
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

50 review citations

Phosphoinositides: tiny lipids with giant impact on cell regulation.
Balla T. (2013)
49 more