› 6nzj

X-ray diffraction
2.4Å resolution

Structural Analysis of a Nitrogenase Iron Protein from Methanosarcina acetivorans: Implications for CO2 Capture by a Surface-Exposed [Fe4S4] Cluster

Released:
DOI: 10.2210/pdb6nzj/pdb
PDB entry 6nzj coloured by chain, front view.
PDB entry 6nzj coloured by chain, side view.
PDB entry 6nzj coloured by chain, top view.
Source organism: Methanosarcina acetivorans C2A
Primary publication:
Structural Analysis of a Nitrogenase Iron Protein from Methanosarcina acetivorans: Implications for CO2 Capture by a Surface-Exposed [Fe4S4] Cluster.
Rettberg LA, Kang W, Stiebritz MT, Hiller CJ, Lee CC, Liedtke J, Ribbe MW, Hu Y
mBio 10 (2019)
PMID: 31289188

Function and Biology Details

Reaction catalysed: 
8 reduced ferredoxin + 8 H(+) + N(2) + 16 ATP + 16 H(2)O = 8 oxidized ferredoxin + H(2) + 2 NH(3) + 16 ADP + 16 phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186281 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrogenase iron protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 273 amino acids
Theoretical weight: 29.27 KDa
Source organism: Methanosarcina acetivorans C2A
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8TJ93 (Residues: 1-273; Coverage: 100%)
Gene names: MA_3895, nifH
Sequence domains: 4Fe-4S iron sulfur cluster binding proteins, NifH/frxC family

Ligands and Environments

2 bound ligands:
Ligand SF4 1 x SF4
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P6522
Unit cell:
a: 96.169Å b: 96.169Å c: 320.244Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.188 0.186 0.216
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Structural Enzymology of Nitrogenase Enzymes.
Einsle et al. (2020)
4 more

4 mentions without citation

The Conversion of Carbon Monoxide and Carbon Dioxide by Nitrogenases.
Oehlmann et al. (2022)
3 more