› 6o11

X-ray diffraction
1.84Å resolution

E. coli cysteine desulfurase SufS C364A with a Cys-aldimine intermediate

Released:
DOI: 10.2210/pdb6o11/pdb
PDB entry 6o11 coloured by chain, front view.
PDB entry 6o11 coloured by chain, side view.
PDB entry 6o11 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Direct observation of intermediates in the SufS cysteine desulfurase reaction reveals functional roles of conserved active-site residues.
Blahut M, Wise CE, Bruno MR, Dong G, Makris TM, Frantom PA, Dunkle JA, Outten FW
J Biol Chem 294 12444-12458 (2019)
PMID: 31248989

Function and Biology Details

Reactions catalysed: 
(1a) L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine
L-selenocysteine + reduced acceptor = selenide + L-alanine + acceptor
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160167 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cysteine desulfurase Chain: A
Molecule details ›
Chain: A
Length: 406 amino acids
Theoretical weight: 44.45 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P77444 (Residues: 1-406; Coverage: 100%)
Gene names: JW1670, b1680, csdB, sufS, ynhB
Sequence domains: Aminotransferase class-V

Ligands and Environments

2 bound ligands:
Ligand CL 1 x CL
Ligand C6P 1 x C6P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P42212
Unit cell:
a: 126.426Å b: 126.426Å c: 67.087Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.184 0.216
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Fe-S cluster biogenesis by the bacterial Suf pathway.
Blahut et al. (2020)
1 more

2 mentions without citation

Direct observation of intermediates in the SufS cysteine desulfurase reaction reveals functional roles of conserved active-site residues.
Blahut et al. (2019)
1 more