› 6o15

X-ray diffraction
1.35Å resolution

Crystal structure of a putative oxidoreductase YjhC from Escherichia coli in complex with NAD(H)

Released:
DOI: 10.2210/pdb6o15/pdb
PDB entry 6o15 coloured by chain, front view.
PDB entry 6o15 coloured by chain, side view.
PDB entry 6o15 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
On the structure and function of Escherichia coli YjhC: An oxidoreductase involved in bacterial sialic acid metabolism.
Horne CR, Kind L, Davies JS, Dobson RCJ
Proteins 88 654-668 (2020)
PMID: 31697432

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153870 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2,7-anhydro-N-acetylneuraminate hydratase Chains: A, B
Molecule details ›
Chains: A, B
Length: 372 amino acids
Theoretical weight: 41.44 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P39353 (Residues: 1-372; Coverage: 100%)
Gene names: JW5769, b4280, nanY, yjhC
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 3 x SO4
Ligand NAD 2 x NAD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 51.514Å b: 84.673Å c: 188.118Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.151 0.15 0.169
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Intramolecular Lactones of Sialic Acids.
Rota et al. (2020)
3 more

3 mentions without citation

Biochemical and structural basis of sialic acid utilization by gut microbes.
Bell et al. (2023)
2 more