› 6o31

X-ray diffraction
1.51Å resolution

CRYSTAL STRUCTURE OF THE ACTIN-BINDING DOMAIN OF HUMAN ALPHA-ACTININ-4

Released:
DOI: 10.2210/pdb6o31/pdb
PDB entry 6o31 coloured by chain, front view.
PDB entry 6o31 coloured by chain, side view.
PDB entry 6o31 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Phosphorylation of ACTN4 Leads to Podocyte Vulnerability and Proteinuric Glomerulosclerosis.
Feng D, Kumar M, Muntel J, Gurley SB, Birrane G, Stillman IE, Ding L, Wang M, Ahmed S, Schlondorff J, Alper SL, Ferrante T, Marquez SL, Ng CF, Novak R, Ingber DE, Steen H, Pollak MR
J Am Soc Nephrol 31 1479-1495 (2020)
PMID: 32540856

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129066 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-actinin-4 Chain: A
Molecule details ›
Chain: A
Length: 234 amino acids
Theoretical weight: 26.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O43707 (Residues: 47-271; Coverage: 25%)
Gene name: ACTN4
Sequence domains: Calponin homology (CH) domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P41212
Unit cell:
a: 38.069Å b: 38.069Å c: 302.655Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.208 0.254
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Abnormal Crosstalk between Endothelial Cells and Podocytes Mediates Tyrosine Kinase Inhibitor (TKI)-Induced Nephrotoxicity.
Gu et al. (2021)
4 more

3 mentions without citation

Phosphorylation of ACTN4 Leads to Podocyte Vulnerability and Proteinuric Glomerulosclerosis.
Feng et al. (2020)
2 more