› 6o3t

X-ray diffraction
3.06Å resolution

Structural basis of FOXC2 and DNA interactions

Released:
DOI: 10.2210/pdb6o3t/pdb
PDB entry 6o3t coloured by chain, front view.
PDB entry 6o3t coloured by chain, side view.
PDB entry 6o3t coloured by chain, top view.
Source organisms:
Primary publication:
Crystal Structure of FOXC2 in Complex with DNA Target.
Li S, Pradhan L, Ashur S, Joshi A, Nam HJ
ACS Omega 4 10906-10914 (2019)
PMID: 31460188

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120364 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Forkhead box protein C2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 95 amino acids
Theoretical weight: 11.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q99958 (Residues: 70-164; Coverage: 19%)
Gene names: FKHL14, FOXC2, MFH1
Sequence domains: Forkhead domain
DNA (5'-D(*AP*AP*AP*TP*TP*GP*TP*TP*TP*AP*TP*AP*AP*AP*CP*AP*GP*CP*CP*CP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 21 nucleotides
Theoretical weight: 6.43 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*TP*TP*CP*GP*GP*GP*CP*TP*GP*TP*TP*TP*AP*TP*AP*AP*AP*CP*AP*AP*T)-3') Chain: D
Molecule details ›
Chain: D
Length: 21 nucleotides
Theoretical weight: 6.45 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 128.365Å b: 41.812Å c: 82.654Å
α: 90° β: 98.3° γ: 90°
R-values:
R R work R free
0.235 0.233 0.268
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

2 review citations

Human FoxP Transcription Factors as Tractable Models of the Evolution and Functional Outcomes of Three-Dimensional Domain Swapping.
Villalobos et al. (2021)
1 more

2 mentions without citation

Toward a mechanistic understanding of DNA binding by forkhead transcription factors and its perturbation by pathogenic mutations.
Dai et al. (2021)
1 more