› 6o5j

X-ray diffraction
1.63Å resolution

Crystal Structure of DAD2 bound to quinazolinone derivative

Released:
DOI: 10.2210/pdb6o5j/pdb
PDB entry 6o5j coloured by chain, front view.
PDB entry 6o5j coloured by chain, side view.
PDB entry 6o5j coloured by chain, top view.
Source organism: Petunia x hybrida
Primary publication:
Chemical synthesis and characterization of a new quinazolinedione competitive antagonist for strigolactone receptors with an unexpected binding mode.
Hamiaux C, Larsen L, Lee HW, Luo Z, Sharma P, Hawkins BC, Perry NB, Snowden KC
Biochem J 476 1843-1856 (2019)
PMID: 31186286

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125406 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable strigolactone esterase DAD2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 264 amino acids
Theoretical weight: 29.43 KDa
Source organism: Petunia x hybrida
Expression system: Escherichia coli
UniProt:
  • Canonical: J9U5U9 (Residues: 3-266; Coverage: 99%)
Gene name: DAD2
Sequence domains: Alpha/beta hydrolase family

Ligands and Environments

4 bound ligands:
Ligand LM7 2 x LM7
Ligand GOL 2 x GOL
Ligand ACT 1 x ACT
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P1
Unit cell:
a: 36.63Å b: 56.83Å c: 68.8Å
α: 95.69° β: 94.59° γ: 108.74°
R-values:
R R work R free
0.146 0.144 0.182
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Molecular basis for high ligand sensitivity and selectivity of strigolactone receptors in Striga.
Wang et al. (2021)
2 more

3 mentions without citation

Review on Structures of Pesticide Targets.
Li et al. (2020)
2 more