6o6k

X-ray diffraction
3.6Å resolution

HUaa 19bp SYM DNA pH 5.5

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-120366 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA-binding protein HU-alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 90 amino acids
Theoretical weight: 9.55 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P0ACF0 (Residues: 1-90; Coverage: 100%)
Gene names: JW3964, b4000, hupA
Sequence domains: Bacterial DNA-binding protein
DNA (5'-D(P*AP*TP*TP*TP*CP*AP*TP*GP*AP*T)-3') Chain: C
Molecule details ›
Chain: C
Length: 10 nucleotides
Theoretical weight: 3.03 KDa
Source organism: Escherichia coli
Expression system: Not provided
DNA (5'-D(P*CP*AP*TP*CP*AP*TP*GP*AP*AP*A)-3') Chain: D
Molecule details ›
Chain: D
Length: 10 nucleotides
Theoretical weight: 3.04 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P42212
Unit cell:
a: 84.79Å b: 84.79Å c: 63.33Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.257 0.252 0.302
Expression systems:
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'
  • Not provided