6o6p

X-ray diffraction
3.85Å resolution

Structure of the regulator FasR from Mycobacterium tuberculosis in complex with DNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-120367 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
HTH tetR-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 248 amino acids
Theoretical weight: 27.29 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O05858 (Residues: 1-228; Coverage: 100%)
Gene name: Rv3208
Sequence domains:
DNA-forward Chain: C
Molecule details ›
Chain: C
Length: 25 nucleotides
Theoretical weight: 7.63 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Not provided
DNA-reverse Chain: D
Molecule details ›
Chain: D
Length: 25 nucleotides
Theoretical weight: 7.73 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: C2221
Unit cell:
a: 61.697Å b: 174.68Å c: 158.169Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.292 0.288 0.332
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided