› 6o6y

X-ray diffraction
1.96Å resolution

Crystal structure of Csm6 in complex with cyclic-tetraadenylates (cA4) by cocrystallization of Csm6 and cA4

Released:
DOI: 10.2210/pdb6o6y/pdb
PDB entry 6o6y coloured by chain, front view.
PDB entry 6o6y coloured by chain, side view.
PDB entry 6o6y coloured by chain, top view.
Source organism: Thermococcus onnurineus
Primary publication:
CRISPR-Cas III-A Csm6 CARF Domain Is a Ring Nuclease Triggering Stepwise cA4 Cleavage with ApA>p Formation Terminating RNase Activity.
Jia N, Jones R, Yang G, Ouerfelli O, Patel DJ
Mol Cell 75 944-956.e6 (2019)
PMID: 31326273

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110432 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CRISPR system endoribonuclease Csx1-like domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 440 amino acids
Theoretical weight: 49.8 KDa
Source organism: Thermococcus onnurineus
Expression system: Escherichia coli
UniProt:
  • Canonical: B6YWC3 (Residues: 1-432; Coverage: 100%)
Gene name: TON_0898
Sequence domains: CRISPR-associated (Cas) DxTHG family

Ligands and Environments

2 bound ligands:
Ligand ACK 2 x ACK
Ligand LQJ 2 x LQJ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2221
Unit cell:
a: 103.451Å b: 163.963Å c: 111.105Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.206 0.255
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Cyclic di-AMP, a second messenger of primary importance: tertiary structures and binding mechanisms.
He et al. (2020)
11 more

6 mentions without citation

Bacterial origins of cyclic nucleotide-activated antiviral immune signaling.
Patel et al. (2022)
5 more