› 6o75

X-ray diffraction
2.6Å resolution

Crystal structure of Csm1-Csm4 cassette in complex with pppApA

Released:
DOI: 10.2210/pdb6o75/pdb
PDB entry 6o75 coloured by chain, front view.
PDB entry 6o75 coloured by chain, side view.
PDB entry 6o75 coloured by chain, top view.
Source organism: Thermococcus onnurineus
Primary publication:
Second Messenger cA4 Formation within the Composite Csm1 Palm Pocket of Type III-A CRISPR-Cas Csm Complex and Its Release Path.
Jia N, Jones R, Sukenick G, Patel DJ
Mol Cell 75 933-943.e6 (2019)
PMID: 31326272

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110427 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
CRISPR system single-strand-specific deoxyribonuclease Cas10/Csm1 (subtype III-A) Chain: A
Molecule details ›
Chain: A
Length: 791 amino acids
Theoretical weight: 89.71 KDa
Source organism: Thermococcus onnurineus
Expression system: Escherichia coli
UniProt:
  • Canonical: B6YWB8 (Residues: 1-777; Coverage: 100%)
Gene names: TON_0893, cas10, csm1
Sequence domains:
CRISPR system Cms protein Csm4 Chain: B
Molecule details ›
Chain: B
Length: 289 amino acids
Theoretical weight: 32.35 KDa
Source organism: Thermococcus onnurineus
Expression system: Escherichia coli
UniProt:
  • Canonical: B6YWC1 (Residues: 1-289; Coverage: 100%)
Gene names: TON_0896, csm4
RNA (5'-D(*(ATP))-R(P*A)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 2 nucleotides
Theoretical weight: 773 Da

Ligands and Environments

1 bound ligand:
Ligand MN 2 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P6522
Unit cell:
a: 156.445Å b: 156.445Å c: 186.415Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.226 0.223 0.271
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Chemistry of Class 1 CRISPR-Cas effectors: Binding, editing, and regulation.
Liu et al. (2020)
5 more

1 mention without citation

Second Messenger cA4 Formation within the Composite Csm1 Palm Pocket of Type III-A CRISPR-Cas Csm Complex and Its Release Path.
Jia et al. (2019)