› 6o8g

X-ray diffraction
2.64Å resolution

Crystal structure of UvrB bound to fully duplex DNA

Released:
DOI: 10.2210/pdb6o8g/pdb
PDB entry 6o8g coloured by chain, front view.
PDB entry 6o8g coloured by chain, side view.
PDB entry 6o8g coloured by chain, top view.
Source organism: [Bacillus] caldotenax
Primary publication:
Mechanism of DNA Lesion Homing and Recognition by the Uvr Nucleotide Excision Repair System.
Lee SJ, Sung RJ, Verdine GL
Research (Wash D C) 2019 5641746 (2019)
PMID: 31549070

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120370 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
UvrABC system protein B Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 593 amino acids
Theoretical weight: 68.16 KDa
Source organism: [Bacillus] caldotenax
Expression system: Escherichia coli
UniProt:
  • Canonical: P56981 (Residues: 2-190, 191-593; Coverage: 90%)
Gene name: uvrB
Sequence domains:
DNA (5'-D(*TP*CP*TP*CP*CP*AP*TP*CP*GP*CP*GP*CP*TP*AP*C)-3') Chains: D, F, H
Molecule details ›
Chains: D, F, H
Length: 15 nucleotides
Theoretical weight: 4.48 KDa
DNA (5'-D(*GP*GP*TP*AP*GP*CP*GP*CP*GP*AP*TP*GP*GP*AP*GP*A)-3') Chains: E, G, I
Molecule details ›
Chains: E, G, I
Length: 16 nucleotides
Theoretical weight: 5.03 KDa

Ligands and Environments

3 bound ligands:
Ligand ADP 3 x ADP
Ligand CL 1 x CL
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P21212
Unit cell:
a: 170.685Å b: 201.181Å c: 62.664Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.22 0.274
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Bacterial DNA excision repair pathways.
Wozniak et al. (2022)
2 more

2 mentions without citation

Mechanism of DNA Lesion Homing and Recognition by the Uvr Nucleotide Excision Repair System.
Lee et al. (2019)
1 more