› 6o9m

Electron Microscopy
4.4Å resolution

Structure of the human apo TFIIH

Released:
DOI: 10.2210/pdb6o9m/pdb
PDB entry 6o9m coloured by chain, front view.
PDB entry 6o9m coloured by chain, side view.
PDB entry 6o9m coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Transcription preinitiation complex structure and dynamics provide insight into genetic diseases.
Yan C, Dodd T, He Y, Tainer JA, Tsutakawa SE, Ivanov I
Nat Struct Mol Biol 26 397-406 (2019)
PMID: 31110295
Related structures: EMD-3802

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-148278 (preferred)
Entry contents:
8 distinct polypeptide molecules
Macromolecules (8 distinct):
General transcription and DNA repair factor IIH helicase subunit XPD Chain: 0
Molecule details ›
Chain: 0
Length: 760 amino acids
Theoretical weight: 87.02 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P18074 (Residues: 1-760; Coverage: 100%)
Gene names: ERCC2, XPD, XPDC
Sequence domains:
General transcription factor IIH subunit 1 Chain: 1
Molecule details ›
Chain: 1
Length: 548 amino acids
Theoretical weight: 62.12 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P32780 (Residues: 1-548; Coverage: 100%)
Gene names: BTF2, GTF2H1
Sequence domains:
General transcription factor IIH subunit 4 Chain: 2
Molecule details ›
Chain: 2
Length: 462 amino acids
Theoretical weight: 52.25 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q92759 (Residues: 1-462; Coverage: 100%)
Gene name: GTF2H4
Sequence domains:
CDK-activating kinase assembly factor MAT1 Chain: 3
Molecule details ›
Chain: 3
Length: 309 amino acids
Theoretical weight: 35.87 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P51948 (Residues: 1-309; Coverage: 100%)
Gene names: CAP35, MAT1, MNAT1, RNF66
Sequence domains:
General transcription factor IIH subunit 3 Chain: 4
Molecule details ›
Chain: 4
Length: 308 amino acids
Theoretical weight: 34.42 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q13889 (Residues: 1-308; Coverage: 100%)
Gene name: GTF2H3
Sequence domains: Transcription factor Tfb4
General transcription factor IIH subunit 5 Chain: 5
Molecule details ›
Chain: 5
Length: 71 amino acids
Theoretical weight: 8.06 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q6ZYL4 (Residues: 1-71; Coverage: 100%)
Gene names: C6orf175, GTF2H5, TTDA
Sequence domains: Transcription factor TFIIH complex subunit Tfb5
General transcription factor IIH subunit 2 Chain: 6
Molecule details ›
Chain: 6
Length: 395 amino acids
Theoretical weight: 44.48 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q13888 (Residues: 1-395; Coverage: 100%)
Gene names: BTF2P44, GTF2H2
Sequence domains:
General transcription and DNA repair factor IIH helicase/translocase subunit XPB Chain: 7
Molecule details ›
Chain: 7
Length: 782 amino acids
Theoretical weight: 89.4 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P19447 (Residues: 1-782; Coverage: 100%)
Gene names: ERCC3, XPB, XPBC
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand SF4 1 x SF4
Ligand ZN 6 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.4Å
Relevant EMDB volumes: EMD-3802

Quick links

Citations

12 review citations

Structure and mechanism of the RNA polymerase II transcription machinery.
Schier et al. (2020)
11 more

3 mentions without citation

Envisioning how the prototypic molecular machine TFIIH functions in transcription initiation and DNA repair.
Tsutakawa et al. (2020)
2 more