› 6oa8

X-ray diffraction
1.37Å resolution

Superfolder Green Fluorescent Protein with 4-cyano-L-phenylalanine at the chromophore (position 66)

Released:
DOI: 10.2210/pdb6oa8/pdb
PDB entry 6oa8 coloured by chain, front view.
PDB entry 6oa8 coloured by chain, side view.
PDB entry 6oa8 coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Structural and spectrophotometric investigation of two unnatural amino-acid altered chromophores in the superfolder green fluorescent protein
Olenginski GM, Piacentini J, Harris DR, Runko N, Papoutsis BM, Alter JR, Hess KR, Brewer SH, Phillips-Piro CM
Acta Crystallogr. D Biol. Crystallogr. (2021)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 237 amino acids
Theoretical weight: 26.91 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli str. K-12 substr. DH10B
UniProt:
  • Canonical: P42212 (Residues: 3-238; Coverage: 98%)
Gene name: GFP
Sequence domains: Green fluorescent protein

Ligands and Environments

3 bound ligands:
Ligand EDO 3 x EDO
Ligand SO4 8 x SO4
Ligand NA 4 x NA
1 modified residue:
Ligand M3V 1 x M3V

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P4122
Unit cell:
a: 55.266Å b: 55.266Å c: 166.483Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.161 0.188
Expression system: Escherichia coli str. K-12 substr. DH10B

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