› 6oab

Electron Microscopy
3.6Å resolution

Cdc48-Npl4 complex processing poly-ubiquitinated substrate in the presence of ADP-BeFx, state 2

Released:
DOI: 10.2210/pdb6oab/pdb
PDB entry 6oab coloured by chain, front view.
PDB entry 6oab coloured by chain, side view.
PDB entry 6oab coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Substrate processing by the Cdc48 ATPase complex is initiated by ubiquitin unfolding.
Twomey EC, Ji Z, Wales TE, Bodnar NO, Ficarro SB, Marto JA, Engen JR, Rapoport TA
Science 365 (2019)
PMID: 31249135
Related structures: EMD-20000

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150557 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cell division control protein 48 Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 835 amino acids
Theoretical weight: 92.11 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P25694 (Residues: 1-835; Coverage: 100%)
Gene names: CDC48, YDL126C
Sequence domains:
poly(alanine) substrate Chain: H
Molecule details ›
Chain: H
Length: 24 amino acids
Theoretical weight: 1.72 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand ADP 10 x ADP
Ligand BEF 8 x BEF
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-20000
Expression system: Escherichia coli

Quick links

Citations

41 review citations

Protein quality control in the secretory pathway.
Sun et al. (2019)
40 more

2 mentions without citation

The Cryo-EM Effect: Structural Biology of Neurodegenerative Disease Proteostasis Factors.
Creekmore et al. (2021)
1 more