› 6oau

X-ray diffraction
2.48Å resolution

Apo Structure of WT Lipoprotein Lipase in Complex with GPIHBP1 Mutant N78D N82D produced in GnTI-deficient HEK293-F cells

Released:
DOI: 10.2210/pdb6oau/pdb
PDB entry 6oau coloured by chain, front view.
PDB entry 6oau coloured by chain, side view.
PDB entry 6oau coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of lipoprotein lipase in complex with GPIHBP1.
Arora R, Nimonkar AV, Baird D, Wang C, Chiu CH, Horton PA, Hanrahan S, Cubbon R, Weldon S, Tschantz WR, Mueller S, Brunner R, Lehr P, Meier P, Ottl J, Voznesensky A, Pandey P, Smith TM, Stojanovic A, Flyer A, Benson TE, Romanowski MJ, Trauger JW
Proc Natl Acad Sci U S A 116 10360-10365 (2019)
PMID: 31072929

Function and Biology Details

Reactions catalysed: 
Phosphatidylcholine + H(2)O = 2-acylglycerophosphocholine + a carboxylate
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139260 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Lipoprotein lipase Chains: A, B
Molecule details ›
Chains: A, B
Length: 448 amino acids
Theoretical weight: 50.47 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P06858 (Residues: 28-475; Coverage: 100%)
Gene names: LIPD, LPL
Sequence domains:
Glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 131 amino acids
Theoretical weight: 14.73 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8IV16 (Residues: 21-151; Coverage: 80%)
Gene names: GPIHBP1, HBP1
Sequence domains: u-PAR/Ly-6 domain

Ligands and Environments

3 bound ligands:
Ligand NAG 4 x NAG
Ligand MLI 1 x MLI
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: C2
Unit cell:
a: 177.48Å b: 97.25Å c: 77.21Å
α: 90° β: 93.47° γ: 90°
R-values:
R R work R free
0.215 0.213 0.246
Expression system: Homo sapiens

Quick links

Citations

14 review citations

GPIHBP1 and Lipoprotein Lipase, Partners in Plasma Triglyceride Metabolism.
Young et al. (2019)
13 more

2 mentions without citation

Current Knowledge on Mammalian Phospholipase A1, Brief History, Structures, Biochemical and Pathophysiological Roles.
Yaginuma et al. (2022)
1 more