› 6obt

X-ray diffraction
1.8Å resolution

Structural insights into dehydratase substrate selection for the borrelidin and fluvirucin polyketide synthases

Released:
DOI: 10.2210/pdb6obt/pdb
PDB entry 6obt coloured by chain, front view.
PDB entry 6obt coloured by chain, side view.
PDB entry 6obt coloured by chain, top view.
Source organism: Streptomyces parvulus
Primary publication:
Structural insights into dehydratase substrate selection for the borrelidin and fluvirucin polyketide synthases.
Barajas JF, McAndrew RP, Thompson MG, Backman TWH, Pang B, de Rond T, Pereira JH, Benites VT, Martín HG, Baidoo EEK, Hillson NJ, Adams PD, Keasling JD
J Ind Microbiol Biotechnol 46 1225-1235 (2019)
PMID: 31115703

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180683 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Borrelidin polyketide synthase, type I Chain: A
Molecule details ›
Chain: A
Length: 314 amino acids
Theoretical weight: 33.76 KDa
Source organism: Streptomyces parvulus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q70I00 (Residues: 2588-2859; Coverage: 8%)
Gene name: borA3
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: C2
Unit cell:
a: 108.21Å b: 63.35Å c: 40.67Å
α: 90° β: 100.44° γ: 90°
R-values:
R R work R free
0.203 0.203 0.232
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Site directed mutagenesis as a precision tool to enable synthetic biology with engineered modular polyketide synthases.
Drufva et al. (2020)
1 more

1 mention without citation

An Unconventional Melanin Biosynthesis Pathway in Ustilago maydis.
Reyes-Fernández et al. (2021)