› 6oc6

X-ray diffraction
2.89Å resolution

Lanthanide-dependent methanol dehydrogenase XoxF from Methylobacterium extorquens, in complex with Lanthanum and Pyrroloquinoline quinone

Released:
DOI: 10.2210/pdb6oc6/pdb
PDB entry 6oc6 coloured by chain, front view.
PDB entry 6oc6 coloured by chain, side view.
PDB entry 6oc6 coloured by chain, top view.
Source organism: Methylorubrum extorquens AM1
Primary publication:
Lanthanide-dependent alcohol dehydrogenases require an essential aspartate residue for metal coordination and enzymatic function.
Good NM, Fellner M, Demirer K, Hu J, Hausinger RP, Martinez-Gomez NC
J Biol Chem 295 8272-8284 (2020)
PMID: 32366463

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111459 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PQQ-dependent dehydrogenase, methanol/ethanol family Chains: A, B
Molecule details ›
Chains: A, B
Length: 601 amino acids
Theoretical weight: 64.97 KDa
Source organism: Methylorubrum extorquens AM1
Expression system: Methylorubrum extorquens AM1
UniProt:
  • Canonical: C5B120 (Residues: 1-601; Coverage: 100%)
Gene names: MexAM1_META1p1740, xoxF
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand LA 2 x LA
Ligand PQQ 2 x PQQ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21212
Unit cell:
a: 101.272Å b: 101.941Å c: 103.691Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.204 0.258
Expression system: Methylorubrum extorquens AM1

Quick links

Citations

4 review citations

Verrucomicrobial methanotrophs: ecophysiology of metabolically versatile acidophiles.
Schmitz et al. (2021)
3 more

2 mentions without citation

Lanthanide-dependent alcohol dehydrogenases require an essential aspartate residue for metal coordination and enzymatic function.
Good et al. (2020)
1 more