› 6od4

X-ray diffraction
1.7Å resolution

Human TCF4 C-terminal bHLH domain in Complex with 11-bp Oligonucleotide Containing E-box Sequence

Released:
DOI: 10.2210/pdb6od4/pdb
PDB entry 6od4 coloured by chain, front view.
PDB entry 6od4 coloured by chain, side view.
PDB entry 6od4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for preferential binding of human TCF4 to DNA containing 5-carboxylcytosine.
Yang J, Horton JR, Li J, Huang Y, Zhang X, Blumenthal RM, Cheng X
Nucleic Acids Res 47 8375-8387 (2019)
PMID: 31081034

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-120379 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Transcription factor 4 Chains: A, B, G, H
Molecule details ›
Chains: A, B, G, H
Length: 62 amino acids
Theoretical weight: 7.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P15884 (Residues: 565-624; Coverage: 9%)
Gene names: BHLHB19, ITF2, SEF2, TCF4
Sequence domains: Helix-loop-helix DNA-binding domain
Structure domains: Helix-loop-helix DNA-binding domain
DNA (5'-D(*TP*AP*CP*AP*CP*GP*TP*GP*TP*A)-3') Chains: W, X
Molecule details ›
Chains: W, X
Length: 11 nucleotides
Theoretical weight: 3.35 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P1
Unit cell:
a: 36.614Å b: 43.602Å c: 43.557Å
α: 97.17° β: 102.56° γ: 102.53°
R-values:
R R work R free
0.224 0.222 0.267
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

6 review citations

Transcription factor 4 and its association with psychiatric disorders.
Teixeira et al. (2021)
5 more

3 mentions without citation

Homodimeric and Heterodimeric Interactions among Vertebrate Basic Helix-Loop-Helix Transcription Factors.
Torres-Machorro AL. (2021)
2 more