6oge

Electron Microscopy
4.36Å resolution

Cryo-EM structure of Her2 extracellular domain-Trastuzumab Fab-Pertuzumab Fab complex

Released:
Source organism: Homo sapiens
Primary publication:
Cryo-EM Structure of HER2-trastuzumab-pertuzumab complex.
PLoS One 14 e0216095 (2019)
PMID: 31042744
Related structures: EMD-20055

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-134614 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (6 distinct):
Receptor tyrosine-protein kinase erbB-2 Chain: A
Molecule details ›
Chain: A
Length: 622 amino acids
Theoretical weight: 68.54 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P04626 (Residues: 23-644; Coverage: 50%)
Gene names: ERBB2, HER2, MLN19, NEU, NGL
Sequence domains:
Immunoglobulin kappa constant Chain: B
Molecule details ›
Chain: B
Length: 214 amino acids
Theoretical weight: 23.55 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P01834 (Residues: 1-107; Coverage: 100%)
Gene name: IGKC
Sequence domains: Immunoglobulin C1-set domain
Immunoglobulin gamma-1 heavy chain Chain: C
Molecule details ›
Chain: C
Length: 222 amino acids
Theoretical weight: 23.67 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P0DOX5 (Residues: 1-222; Coverage: 49%)
Sequence domains:
Immunoglobulin kappa constant Chain: D
Molecule details ›
Chain: D
Length: 214 amino acids
Theoretical weight: 23.47 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P01834 (Residues: 1-107; Coverage: 100%)
Gene name: IGKC
Sequence domains: Immunoglobulin C1-set domain
Ig-like domain-containing protein Chain: E
Molecule details ›
Chain: E
Length: 220 amino acids
Theoretical weight: 23.43 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: Q6GMX6 (Residues: 20-71, 72-116, 117-118, 119-235; Coverage: 48%)
Gene name: IGH@
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.36Å
Relevant EMDB volumes: EMD-20055
Expression systems:
  • Homo sapiens
  • Cricetulus griseus