› 6ohu

X-ray diffraction
3.53Å resolution

Structure of EBP and tamoxifen

Released:
DOI: 10.2210/pdb6ohu/pdb
PDB entry 6ohu coloured by chain, front view.
PDB entry 6ohu coloured by chain, side view.
PDB entry 6ohu coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for human sterol isomerase in cholesterol biosynthesis and multidrug recognition.
Long T, Hassan A, Thompson BM, McDonald JG, Wang J, Li X
Nat Commun 10 2452 (2019)
PMID: 31165728

Function and Biology Details

Reaction catalysed: 
5-alpha-cholest-7-en-3-beta-ol = 5-alpha-cholest-8-en-3-beta-ol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172224 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 238 amino acids
Theoretical weight: 27.42 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q15125 (Residues: 2-230; Coverage: 100%)
Gene name: EBP
Sequence domains: EXPERA (EXPanded EBP superfamily)

Ligands and Environments

1 bound ligand:
Ligand CTX 3 x CTX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21212
Unit cell:
a: 192.837Å b: 66.829Å c: 98.678Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.278 0.272 0.326
Expression system: Homo sapiens

Quick links

Citations

5 review citations

TM6SF2: A Novel Genetic Player in Nonalcoholic Fatty Liver and Cardiovascular Disease.
Luo et al. (2022)
4 more

2 mentions without citation

Identification of Four Amoebicidal Nontoxic Compounds by a Molecular Docking Screen of Naegleria fowleri Sterol Δ8-Δ7-Isomerase and Phenotypic Assays.
Shi et al. (2019)
1 more