› 6oi4

X-ray diffraction
1.76Å resolution

RPN13 (19-132)-RPN2 (940-952) pY950-Ub complex

Released:
DOI: 10.2210/pdb6oi4/pdb
PDB entry 6oi4 coloured by chain, front view.
PDB entry 6oi4 coloured by chain, side view.
PDB entry 6oi4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Phosphorylation of Tyr-950 in the proteasome scaffolding protein RPN2 modulates its interaction with the ubiquitin receptor RPN13.
Hemmis CW, Heard SC, Hill CP
J Biol Chem 294 9659-9665 (2019)
PMID: 31064842

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-143378 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Proteasomal ubiquitin receptor ADRM1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 113 amino acids
Theoretical weight: 13.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q16186 (Residues: 20-132; Coverage: 28%)
Gene names: ADRM1, GP110
Sequence domains: Proteasome complex subunit Rpn13, Pru domain
Structure domains: Proteasomal ubiquitin receptor Rpn13/ADRM1
Ubiquitin Chains: C, D
Molecule details ›
Chains: C, D
Length: 76 amino acids
Theoretical weight: 8.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CG48 (Residues: 609-684; Coverage: 11%)
Gene name: UBC
Sequence domains: Ubiquitin family
26S proteasome non-ATPase regulatory subunit 1 Chains: E, F
Molecule details ›
Chains: E, F
Length: 14 amino acids
Theoretical weight: 1.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99460 (Residues: 940-952; Coverage: 1%)
Gene name: PSMD1

Ligands and Environments

1 bound ligand:
Ligand EDO 9 x EDO
1 modified residue:
Ligand PTR 2 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P31
Unit cell:
a: 99.373Å b: 99.373Å c: 41.705Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.174 0.173 0.207
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli

Quick links

Citations

1 review citation

Proteasome interaction with ubiquitinated substrates: from mechanisms to therapies.
Chen et al. (2021)
9 other articles

1 mention without citation

Phosphorylation of Tyr-950 in the proteasome scaffolding protein RPN2 modulates its interaction with the ubiquitin receptor RPN13.
Hemmis et al. (2019)