› 6ok2

X-ray diffraction
3.29Å resolution

PilT4 from Geobacter metallireducens bound to ADP: C3ocococ conformation

Released:
DOI: 10.2210/pdb6ok2/pdb
PDB entry 6ok2 coloured by chain, front view.
PDB entry 6ok2 coloured by chain, side view.
PDB entry 6ok2 coloured by chain, top view.
Source organism: Geobacter metallireducens GS-15
Primary publication:
Multiple conformations facilitate PilT function in the type IV pilus.
McCallum M, Benlekbir S, Nguyen S, Tammam S, Rubinstein JL, Burrows LL, Howell PL
Nat Commun 10 5198 (2019)
PMID: 31729381

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174486 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacterial type II secretion system protein E domain-containing protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 385 amino acids
Theoretical weight: 42.88 KDa
Source organism: Geobacter metallireducens GS-15
Expression system: Escherichia coli
UniProt:
  • Canonical: Q39VU6 (Residues: 1-365; Coverage: 100%)
Gene names: Gmet_1394, pilT-4
Sequence domains: Type II/IV secretion system protein

Ligands and Environments

1 bound ligand:
Ligand ADP 6 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS-II BEAMLINE 17-ID-1
Spacegroup: P212121
Unit cell:
a: 111.817Å b: 120.956Å c: 185.915Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.215 0.256
Expression system: Escherichia coli

Quick links

Citations

8 citation in other articles

PilT and PilU are homohexameric ATPases that coordinate to retract type IVa pili.
Chlebek et al. (2019)
7 more