› 6om4

X-ray diffraction
1.7Å resolution

The structure of Microcin C7 biosynthetic enzyme MccB in complex with N-formylated MccA

Released:
DOI: 10.2210/pdb6om4/pdb
PDB entry 6om4 coloured by chain, front view.
PDB entry 6om4 coloured by chain, side view.
PDB entry 6om4 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Biosynthesis of the RiPP trojan horse nucleotide antibiotic microcin C is directed by the N-formyl of the peptide precursor.
Dong SH, Kulikovsky A, Zukher I, Estrada P, Dubiley S, Severinov K, Nair SK
Chem Sci 10 2391-2395 (2019)
PMID: 30881667

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-167207 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
THIF-type NAD/FAD binding fold domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 348 amino acids
Theoretical weight: 38.85 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q47506 (Residues: 1-348; Coverage: 99%)
Gene name: mccB
Sequence domains: ThiF family
Structure domains:
Microcin C7 Chains: C, D
Molecule details ›
Chains: C, D
Length: 7 amino acids
Theoretical weight: 793 Da
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

4 bound ligands:
Ligand ZN 2 x ZN
Ligand MG 4 x MG
Ligand POP 2 x POP
Ligand ND7 2 x ND7
1 modified residue:
Ligand FME 2 x FME

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P212121
Unit cell:
a: 55.094Å b: 76.059Å c: 131.902Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.157 0.155 0.199
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

4 review citations

Genome mining methods to discover bioactive natural products.
Bauman et al. (2021)
3 more

4 mentions without citation

New developments in RiPP discovery, enzymology and engineering.
Montalbán-López et al. (2021)
3 more