› 6omb

Electron Microscopy
3.7Å resolution

Cdc48 Hexamer (Subunits A to E) with substrate bound to the central pore

Released:
DOI: 10.2210/pdb6omb/pdb
PDB entry 6omb coloured by chain, front view.
PDB entry 6omb coloured by chain, side view.
PDB entry 6omb coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structure of the Cdc48 segregase in the act of unfolding an authentic substrate.
Cooney I, Han H, Stewart MG, Carson RH, Hansen DT, Iwasa JH, Price JC, Hill CP, Shen PS
Science 365 502-505 (2019)
PMID: 31249134
Related structures: EMD-20124

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150558 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cell division control protein 48 Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 835 amino acids
Theoretical weight: 92.11 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P25694 (Residues: 1-835; Coverage: 100%)
Gene names: CDC48, YDL126C
Sequence domains:
Substrate of Cdc48 Chain: G
Molecule details ›
Chain: G
Length: 22 amino acids
Theoretical weight: 1.89 KDa
Source organism: Saccharomyces cerevisiae S288C

Ligands and Environments

3 bound ligands:
Ligand ADP 10 x ADP
Ligand BEF 8 x BEF
Ligand MG 8 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.7Å
Relevant EMDB volumes: EMD-20124

Quick links

Citations

30 review citations

The molecular principles governing the activity and functional diversity of AAA+ proteins.
Puchades et al. (2020)
29 more

1 mention without citation

Structure of the Cdc48 segregase in the act of unfolding an authentic substrate.
Cooney et al. (2019)