› 6on0

X-ray diffraction
1.6Å resolution

STRUCTURE OF N15 CRO COMPLEXED WITH CONSENSUS OPERATOR DNA

Released:
DOI: 10.2210/pdb6on0/pdb
PDB entry 6on0 coloured by chain, front view.
PDB entry 6on0 coloured by chain, side view.
PDB entry 6on0 coloured by chain, top view.
Source organism: Escherichia virus N15
Primary publication:
Extreme divergence between one-to-one orthologs: the structure of N15 Cro bound to operator DNA and its relationship to the λ Cro complex.
Hall BM, Roberts SA, Cordes MHJ
Nucleic Acids Res 47 7118-7129 (2019)
PMID: 31180482

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120397 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Repressor protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 71 amino acids
Theoretical weight: 7.84 KDa
Source organism: Escherichia virus N15
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q37964 (Residues: 1-71; Coverage: 100%)
Gene name: cro
Sequence domains: DNA-binding transcriptional regulator Cro
Structure domains: lambda repressor-like DNA-binding domains
DNA (5'-D(*TP*TP*TP*AP*TP*AP*GP*CP*TP*AP*GP*CP*TP*AP*TP*AP*A)-3') Chains: C, N
Molecule details ›
Chains: C, N
Length: 17 nucleotides
Theoretical weight: 5.2 KDa
Source organism: Escherichia virus N15
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P41212
Unit cell:
a: 56.64Å b: 56.64Å c: 195.93Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.206 0.217
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

1 citations in other articles

Molecular Characterization and Comparative Genomic Analysis of vB_PaeP_YA3, a Novel Temperate Bacteriophage of Pseudomonas aeruginosa.
Yu et al. (2020)

1 mention without citation

Extreme divergence between one-to-one orthologs: the structure of N15 Cro bound to operator DNA and its relationship to the λ Cro complex.
Hall et al. (2019)