› 6or3

X-ray diffraction
1.45Å resolution

Structure of an Acyl Intermediate of Thermomyces Lanuginosa Lipase With Palmitic Acid in an Orthorhombic Crystal

Released:
DOI: 10.2210/pdb6or3/pdb
PDB entry 6or3 coloured by chain, front view.
PDB entry 6or3 coloured by chain, side view.
PDB entry 6or3 coloured by chain, top view.
Source organism: Thermomyces lanuginosus
Primary publication:
The crystal Structures of Thermomyces (Humicola) lanuginosa lipase in complex with enzymatic reactants
McPherson A, Larson SB, Kalasky A
Current Enzyme Inhibition 16 (2020)

Function and Biology Details

Reaction catalysed: 
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-129890 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lipase Chain: A
Molecule details ›
Chain: A
Length: 291 amino acids
Theoretical weight: 31.84 KDa
Source organism: Thermomyces lanuginosus
UniProt:
  • Canonical: O59952 (Residues: 1-291; Coverage: 100%)
Gene name: LIP
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand CA 1 x CA
Ligand PLM 1 x PLM
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: I222
Unit cell:
a: 69.865Å b: 85.268Å c: 86.741Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.161 0.211

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