› 6osh

X-ray diffraction
1.12Å resolution

Potent and Selective Antitumor Antibody Targeting a Membrane-Proximal Epitope of ROR2

Released:
DOI: 10.2210/pdb6osh/pdb
PDB entry 6osh coloured by chain, front view.
PDB entry 6osh coloured by chain, side view.
PDB entry 6osh coloured by chain, top view.
Source organisms:
Primary publication:
Affinity maturation, humanization, and co-crystallization of a rabbit anti-human ROR2 monoclonal antibody for therapeutic applications.
Goydel RS, Weber J, Peng H, Qi J, Soden J, Freeth J, Park H, Rader C
J Biol Chem 295 5995-6006 (2020)
PMID: 32193207

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-232125 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Antibody Light chain variable region Chain: L
Molecule details ›
Chain: L
Length: 113 amino acids
Theoretical weight: 11.96 KDa
Source organism: Oryctolagus cuniculus
Expression system: Escherichia coli
Tyrosine-protein kinase transmembrane receptor ROR2 Chain: K
Molecule details ›
Chain: K
Length: 96 amino acids
Theoretical weight: 10.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q01974 (Residues: 314-394; Coverage: 9%)
Gene names: NTRKR2, ROR2
Sequence domains: Kringle domain
Antibody heavy chain variable region Chain: H
Molecule details ›
Chain: H
Length: 114 amino acids
Theoretical weight: 12.37 KDa
Source organism: Oryctolagus cuniculus
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 45.165Å b: 67.201Å c: 89.61Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.162 0.162 0.169
Expression system: Escherichia coli

Quick links

Citations

2 review citations

The WNT/ROR Pathway in Cancer: From Signaling to Therapeutic Intervention.
Menck et al. (2021)
1 more

2 mentions without citation

Affinity maturation, humanization, and co-crystallization of a rabbit anti-human ROR2 monoclonal antibody for therapeutic applications.
Goydel et al. (2020)
1 more