6oua

Electron Microscopy
4.18Å resolution

Cryo-EM structure of the yeast Ctf3 complex

Released:
Source organism: Saccharomyces cerevisiae
Primary publication:
The structure of the yeast Ctf3 complex.
Elife 8 (2019)
PMID: 31194673
Related structures: EMD-20200

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155608 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Inner kinetochore subunit CTF3 Chain: I
Molecule details ›
Chain: I
Length: 736 amino acids
Theoretical weight: 84.62 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12748 (Residues: 1-733; Coverage: 100%)
Gene names: CHL3, CTF3, YLR381W
Inner kinetochore subunit MCM16 Chain: H
Molecule details ›
Chain: H
Length: 184 amino acids
Theoretical weight: 21.44 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12262 (Residues: 1-181; Coverage: 100%)
Gene names: MCM16, YPR046W
Sequence domains: CENPH protein
Inner kinetochore subunit MCM22 Chain: K
Molecule details ›
Chain: K
Length: 242 amino acids
Theoretical weight: 27.87 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P47167 (Residues: 1-239; Coverage: 100%)
Gene names: J2122, MCM22, YJR135C

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.18Å
Relevant EMDB volumes: EMD-20200
Expression system: Escherichia coli