› 6oug

X-ray diffraction
3.01Å resolution

Structure of drug-resistant V27A mutant of the influenza M2 proton channel bound to spiroadamantyl amine inhibitor, TM + cytosolic helix construct

Released:
DOI: 10.2210/pdb6oug/pdb
PDB entry 6oug coloured by chain, front view.
PDB entry 6oug coloured by chain, side view.
PDB entry 6oug coloured by chain, top view.
Source organism: Influenza A virus (A/Memphis/1/1971(H3N2))
Primary publication:
X-ray Crystal Structures of the Influenza M2 Proton Channel Drug-Resistant V27A Mutant Bound to a Spiro-Adamantyl Amine Inhibitor Reveal the Mechanism of Adamantane Resistance.
Thomaston JL, Konstantinidi A, Liu L, Lambrinidis G, Tan J, Caffrey M, Wang J, Degrado WF, Kolocouris A
Biochemistry 59 627-634 (2020)
PMID: 31894969

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174897 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Matrix protein 2 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 41 amino acids
Theoretical weight: 4.78 KDa
Source organism: Influenza A virus (A/Memphis/1/1971(H3N2))
Expression system: Not provided
UniProt:
  • Canonical: Q3YPZ4 (Residues: 21-61; Coverage: 42%)
Gene name: M
Sequence domains: Influenza Matrix protein (M2)

Ligands and Environments

1 bound ligand:
Ligand E01 2 x E01
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21212
Unit cell:
a: 49.42Å b: 49.38Å c: 122.38Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.285 0.273 0.301
Expression system: Not provided

Quick links

Citations

3 review citations

Put a cork in it: Plugging the M2 viral ion channel to sink influenza.
Jalily et al. (2020)
2 more

2 mentions without citation

From Acid Activation Mechanisms of Proton Conduction to Design of Inhibitors of the M2 Proton Channel of Influenza A Virus.
Aledavood et al. (2021)
1 more