› 6owo

Electron Microscopy
3.2Å resolution

CRYO-EM STRUCTURE OF PHOSPHORYLATED AP-2 CORE BOUND TO NECAP

Released:
DOI: 10.2210/pdb6owo/pdb
PDB entry 6owo coloured by chain, front view.
PDB entry 6owo coloured by chain, side view.
PDB entry 6owo coloured by chain, top view.
Source organisms:
Primary publication:
A structural mechanism for phosphorylation-dependent inactivation of the AP2 complex.
Partlow EA, Baker RW, Beacham GM, Chappie JS, Leschziner AE, Hollopeter G
Elife 8 (2019)
PMID: 31464684
Related structures: EMD-20215

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-148061 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
AP-2 complex subunit alpha-2 Chain: A
Molecule details ›
Chain: A
Length: 621 amino acids
Theoretical weight: 69.66 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P17427 (Residues: 1-271, 272-620; Coverage: 66%)
Gene names: Adtab, Ap2a2
Sequence domains: Adaptin N terminal region
AP-2 complex subunit beta Chain: B
Molecule details ›
Chain: B
Length: 591 amino acids
Theoretical weight: 66.95 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9DBG3 (Residues: 1-591; Coverage: 63%)
Gene names: Ap2b1, Clapb1
Sequence domains: Adaptin N terminal region
AP-2 complex subunit mu Chain: M
Molecule details ›
Chain: M
Length: 435 amino acids
Theoretical weight: 49.81 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P84091 (Residues: 1-435; Coverage: 100%)
Gene names: Ap2m1, Clapm1
Sequence domains:
AP-2 complex subunit sigma Chain: S
Molecule details ›
Chain: S
Length: 142 amino acids
Theoretical weight: 17.04 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P62744 (Residues: 1-142; Coverage: 100%)
Gene names: Ap17, Ap2s1, Claps2
Sequence domains: Clathrin adaptor complex small chain
Adaptin ear-binding coat-associated protein 2 Chain: N
Molecule details ›
Chain: N
Length: 266 amino acids
Theoretical weight: 28.63 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9D1J1 (Residues: 1-266; Coverage: 100%)
Gene name: Necap2
Sequence domains: Protein of unknown function (DUF1681)

Ligands and Environments

No bound ligands
1 modified residue:
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-20215
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Endocytosis in the adaptation to cellular stress.
López-Hernández et al. (2020)
4 other articles

2 mentions without citation

A structural mechanism for phosphorylation-dependent inactivation of the AP2 complex.
Partlow et al. (2019)
1 more