› 6oyc

X-ray diffraction
2.35Å resolution

Glycosylation Associate Protein (Gap123) complex from Streptococcus agalactiae

Released:
DOI: 10.2210/pdb6oyc/pdb
PDB entry 6oyc coloured by chain, front view.
PDB entry 6oyc coloured by chain, side view.
PDB entry 6oyc coloured by chain, top view.
Source organism: Streptococcus agalactiae 2603V/R
Entry authors: zhang H, Wu H

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-184271 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Accessory secretory protein Asp1 Chain: A
Molecule details ›
Chain: A
Length: 514 amino acids
Theoretical weight: 60.54 KDa
Source organism: Streptococcus agalactiae 2603V/R
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8DYM4 (Residues: 1-514; Coverage: 100%)
Gene name: SAG1452
Sequence domains: Accessory Sec system protein Asp1
Accessory Sec system protein Asp2 Chain: B
Molecule details ›
Chain: B
Length: 519 amino acids
Theoretical weight: 59.25 KDa
Source organism: Streptococcus agalactiae 2603V/R
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8DYM5 (Residues: 1-494; Coverage: 100%)
Gene name: SAG1451
Sequence domains: Accessory Sec system GspB-transporter
Conserved domain protein Chain: C
Molecule details ›
Chain: C
Length: 330 amino acids
Theoretical weight: 37.93 KDa
Source organism: Streptococcus agalactiae 2603V/R
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8DYM6 (Residues: 1-330; Coverage: 100%)
Gene name: SAG1450
Sequence domains: Accessory Sec secretory system ASP3

Ligands and Environments

2 bound ligands:
Ligand GOL 13 x GOL
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21212
Unit cell:
a: 103.009Å b: 168.321Å c: 100.034Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.181 0.227
Expression system: Escherichia coli BL21(DE3)

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