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› 6oyv

X-ray diffraction
3.1Å resolution

Structure of an ancestral-reconstructed cytochrome P450 1B1 with estradiol

Released:
DOI: 10.2210/pdb6oyv/pdb
Model geometry
Fit model/data
PDB entry 6oyv coloured by chain, front view.
PDB entry 6oyv coloured by chain, side view.
PDB entry 6oyv coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Structure of an ancestral mammalian family 1B1 cytochrome P450 with increased thermostability.
Bart AG, Harris KL, Gillam EMJ, Scott EE
J Biol Chem 295 5640-5653 (2020)
PMID: 32156703

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-167225 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P450 1B1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 493 amino acids
Theoretical weight: 55.35 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand EST 2 x EST
Ligand HEM 2 x HEM
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: I4
Unit cell:
a: 162.362Å b: 162.362Å c: 95.694Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.246 0.243 0.276
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Engineering functional thermostable proteins using ancestral sequence reconstruction.
Thomson et al. (2022)
1 more

3 mentions without citation

Application of Various Molecular Modelling Methods in the Study of Estrogens and Xenoestrogens.
Mazurek et al. (2020)
2 more