› 6oze

X-ray diffraction
1.5Å resolution

Crystal structure of the catalytic domain of human Endonuclease V (C140S/C225S/C226A/C228S)

Released:
DOI: 10.2210/pdb6oze/pdb
PDB entry 6oze coloured by chain, front view.
PDB entry 6oze coloured by chain, side view.
PDB entry 6oze coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Evolution of Inosine-Specific Endonuclease V from Bacterial DNase to Eukaryotic RNase.
Wu J, Samara NL, Kuraoka I, Yang W
Mol Cell 76 44-56.e3 (2019)
PMID: 31444105

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185437 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endonuclease V Chain: A
Molecule details ›
Chain: A
Length: 246 amino acids
Theoretical weight: 27.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8N8Q3 (Residues: 9-254; Coverage: 87%)
Gene name: ENDOV
Sequence domains: Endonuclease V

Ligands and Environments

3 bound ligands:
Ligand ACT 2 x ACT
Ligand GOL 2 x GOL
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P31
Unit cell:
a: 50.949Å b: 50.949Å c: 102.978Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.173 0.172 0.191
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Function and Molecular Mechanism of the DNA Damage Response in Immunity and Cancer Immunotherapy.
Ye et al. (2021)
5 more

1 mention without citation

Evolution of Inosine-Specific Endonuclease V from Bacterial DNase to Eukaryotic RNase.
Wu et al. (2019)