› 6p18

Electron Microscopy
3.5Å resolution

Q21 transcription antitermination complex: loading complex

Released:
DOI: 10.2210/pdb6p18/pdb
PDB entry 6p18 coloured by chain, front view.
PDB entry 6p18 coloured by chain, side view.
PDB entry 6p18 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural basis of Q-dependent antitermination.
Yin Z, Kaelber JT, Ebright RH
Proc Natl Acad Sci U S A 116 18384-18390 (2019)
PMID: 31455742
Related structures: EMD-20233

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120417 (preferred)
Entry contents:
6 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (9 distinct):
DNA-directed RNA polymerase subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 329 amino acids
Theoretical weight: 36.56 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0A7Z4 (Residues: 1-329; Coverage: 100%)
Gene names: JW3257, b3295, pez, phs, rpoA, sez
Sequence domains:
DNA-directed RNA polymerase subunit beta Chain: C
Molecule details ›
Chain: C
Length: 1342 amino acids
Theoretical weight: 150.82 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0A8V2 (Residues: 1-1342; Coverage: 100%)
Gene names: JW3950, b3987, groN, nitB, rif, ron, rpoB, stl, stv, tabD
Sequence domains:
DNA-directed RNA polymerase subunit beta' Chain: D
Molecule details ›
Chain: D
Length: 1430 amino acids
Theoretical weight: 158.31 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A8T7 (Residues: 1-1407; Coverage: 100%)
Gene names: JW3951, b3988, rpoC, tabB
Sequence domains:
DNA-directed RNA polymerase subunit omega Chain: E
Molecule details ›
Chain: E
Length: 91 amino acids
Theoretical weight: 10.25 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A800 (Residues: 1-91; Coverage: 100%)
Gene names: JW3624, b3649, rpoZ
Sequence domains: RNA polymerase Rpb6
RNA polymerase sigma factor RpoD Chain: F
Molecule details ›
Chain: F
Length: 627 amino acids
Theoretical weight: 71.84 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P00579 (Residues: 1-613; Coverage: 100%)
Gene names: JW3039, alt, b3067, rpoD
Sequence domains:
Q protein Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 162 amino acids
Theoretical weight: 18.67 KDa
UniProt:
  • Canonical: Q9XJQ6 (Residues: 2-23, 24-161; Coverage: 99%)
Gene name: Q
Sequence domains: Phage antitermination protein Q
DNA (67-MER) fragment carrying phage-21 pR' promoter and pause element, nontemplate strand Chain: 1
Molecule details ›
Chain: 1
Length: 67 nucleotides
Theoretical weight: 20.7 KDa
DNA (67-MER) fragment carrying phage-21 pR' promoter and pause element, template strand Chain: 2
Molecule details ›
Chain: 2
Length: 67 nucleotides
Theoretical weight: 20.59 KDa
RNA (5'-R(P*UP*GP*GP*GP*AP*GP*AP*GP*GP*UP*A)-3') Chain: R
Molecule details ›
Chain: R
Length: 11 nucleotides
Theoretical weight: 3.63 KDa

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-20233
Expression systems:
  • Escherichia coli BL21
  • Escherichia coli

Quick links

Citations

4 review citations

Coupled Transcription-Translation in Prokaryotes: An Old Couple With New Surprises.
Irastortza-Olaziregi et al. (2020)
3 more

1 mention without citation

Structural basis of Q-dependent antitermination.
Yin et al. (2019)