› 6p2k

X-ray diffraction
2.15Å resolution

Crystal structure of AFV00434, an ancestral GH74 enzyme

Released:
DOI: 10.2210/pdb6p2k/pdb
PDB entry 6p2k coloured by chain, front view.
PDB entry 6p2k coloured by chain, side view.
PDB entry 6p2k coloured by chain, top view.
Source organism: Simiduia agarivorans SA1 = DSM 21679
Primary publication:
Substrate specificity, regiospecificity, and processivity in glycoside hydrolase family 74.
Arnal G, Stogios PJ, Asohan J, Attia MA, Skarina T, Viborg AH, Henrissat B, Savchenko A, Brumer H
J Biol Chem 294 13233-13247 (2019)
PMID: 31324716

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125534 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fibronectin type-III domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 779 amino acids
Theoretical weight: 85.37 KDa
Source organism: Simiduia agarivorans SA1 = DSM 21679
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: K4KQN2 (Residues: 18-796; Coverage: 55%)
Gene name: M5M_16515

Ligands and Environments

5 bound ligands:
Ligand ZN 6 x ZN
Ligand ACT 1 x ACT
Ligand GOL 14 x GOL
Ligand PE3 6 x PE3
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P213
Unit cell:
a: 173.427Å b: 173.427Å c: 173.427Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.155 0.154 0.204
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Cell Surface Xyloglucan Recognition and Hydrolysis by the Human Gut Commensal Bacteroides uniformis.
Grondin et al. (2022)
1 more

1 mention without citation

Substrate specificity, regiospecificity, and processivity in glycoside hydrolase family 74.
Arnal et al. (2019)