› 6p67

X-ray diffraction
2.9Å resolution

Crystal Structure of a Complex of human IL-7Ralpha with an anti-IL-7Ralpha 2B8 Fab

Released:
DOI: 10.2210/pdb6p67/pdb
PDB entry 6p67 coloured by chain, front view.
PDB entry 6p67 coloured by chain, side view.
PDB entry 6p67 coloured by chain, top view.
Source organisms:
Primary publication:
New anti-IL-7Rα monoclonal antibodies show efficacy against T cell acute lymphoblastic leukemia in pre-clinical models.
Hixon JA, Andrews C, Kashi L, Kohnhorst CL, Senkevitch E, Czarra K, Barata JT, Li W, Schneider JP, Walsh STR, Durum SK
Leukemia 34 35-49 (2020)
PMID: 31439943

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-231766 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (5 distinct):
Anti-IL-7R 2B8 Fab heavy chain Chains: A, C, E, H
Molecule details ›
Chains: A, C, E, H
Length: 225 amino acids
Theoretical weight: 24.27 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
anti-IL-7R 2B8 Fab light chain Chains: B, D, F, L
Molecule details ›
Chains: B, D, F, L
Length: 214 amino acids
Theoretical weight: 23.61 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
Interleukin-7 receptor subunit alpha Chains: G, I, J, K
Molecule details ›
Chains: G, I, J, K
Length: 223 amino acids
Theoretical weight: 25.69 KDa
Source organism: Homo sapiens
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: P16871 (Residues: 21-239; Coverage: 50%)
Gene name: IL7R
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand FUC 1 x FUC
Ligand NAG 4 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P21
Unit cell:
a: 84.9Å b: 219.68Å c: 90.35Å
α: 90° β: 104.328° γ: 90°
R-values:
R R work R free
0.249 0.249 0.266
Expression systems:
  • Homo sapiens
  • Drosophila melanogaster

Quick links

Citations

12 review citations

Facts and Challenges in Immunotherapy for T-Cell Acute Lymphoblastic Leukemia.
Bayón-Calderón et al. (2020)
11 more

1 mention without citation

Modulation of Signaling Mediated by TSLP and IL-7 in Inflammation, Autoimmune Diseases, and Cancer.
Marković et al. (2020)