6p7x

Electron Microscopy
4.3Å resolution

Structure of the K. lactis CBF3 core - Ndc10 D1D2 complex

Released:
Source organism: Kluyveromyces lactis
Primary publication:
Structure of the Centromere Binding Factor 3 Complex from Kluyveromyces lactis.
J Mol Biol 431 4444-4454 (2019)
PMID: 31425683
Related structures: EMD-20272

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-131699 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
KLLA0E03807p Chain: E
Molecule details ›
Chain: E
Length: 407 amino acids
Theoretical weight: 47.05 KDa
Source organism: Kluyveromyces lactis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6CPM4 (Residues: 1-403; Coverage: 55%)
Gene name: KLLA0_E03807g
Sequence domains: Centromere DNA-binding protein complex CBF3 subunit, domain 2
Ctf13 Chain: C
Molecule details ›
Chain: C
Length: 389 amino acids
Theoretical weight: 46.16 KDa
Source organism: Kluyveromyces lactis
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q6CK37 (Residues: 1-389; Coverage: 100%)
Gene name: KLLA0_F13816g
E3 ubiquitin ligase complex SCF subunit Chain: D
Molecule details ›
Chain: D
Length: 182 amino acids
Theoretical weight: 21.08 KDa
Source organism: Kluyveromyces lactis
Expression system: Trichoplusia ni
UniProt:
  • Canonical: O94228 (Residues: 1-182; Coverage: 100%)
Gene name: SKP1
Sequence domains:
Zn(2)-C6 fungal-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 634 amino acids
Theoretical weight: 74.17 KDa
Source organism: Kluyveromyces lactis
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q6CRD4 (Residues: 1-634; Coverage: 100%)
Gene name: KLLA0_D09977g
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.3Å
Relevant EMDB volumes: EMD-20272
Expression systems:
  • Escherichia coli
  • Trichoplusia ni