› 6p8e

X-ray diffraction
2.3Å resolution

Crystal structure of CDK4 in complex with CyclinD1 and P27

Released:
DOI: 10.2210/pdb6p8e/pdb
PDB entry 6p8e coloured by chain, front view.
PDB entry 6p8e coloured by chain, side view.
PDB entry 6p8e coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
p27 allosterically activates cyclin-dependent kinase 4 and antagonizes palbociclib inhibition.
Guiley KZ, Stevenson JW, Lou K, Barkovich KJ, Kumarasamy V, Wijeratne TU, Bunch KL, Tripathi S, Knudsen ES, Witkiewicz AK, Shokat KM, Rubin SM
Science 366 (2019)
PMID: 31831640

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145931 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
G1/S-specific cyclin-D1 Chain: A
Molecule details ›
Chain: A
Length: 249 amino acids
Theoretical weight: 28.45 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P24385 (Residues: 19-267; Coverage: 84%)
Gene names: BCL1, CCND1, PRAD1
Sequence domains:
Cyclin-dependent kinase 4 Chain: B
Molecule details ›
Chain: B
Length: 302 amino acids
Theoretical weight: 33.95 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P11802 (Residues: 2-303; Coverage: 99%)
Gene name: CDK4
Sequence domains: Protein kinase domain
Cyclin-dependent kinase inhibitor 1B Chain: C
Molecule details ›
Chain: C
Length: 72 amino acids
Theoretical weight: 8.77 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P46527 (Residues: 25-93; Coverage: 35%)
Gene names: CDKN1B, KIP1, p27
Sequence domains: Cyclin-dependent kinase inhibitor

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Unit cell:
a: 62.41Å b: 67.49Å c: 187.28Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.172 0.222
Expression system: Spodoptera frugiperda

Quick links

Citations

32 review citations

Cell cycle control in cancer.
Matthews et al. (2022)
31 more

8 mentions without citation

p27 allosterically activates cyclin-dependent kinase 4 and antagonizes palbociclib inhibition.
Guiley et al. (2019)
7 more