› 6pag

X-ray diffraction
2.5Å resolution

Killer cell immunoglobulin-like receptor 2DL3 in complex with HLA-C*07:02

Released:
DOI: 10.2210/pdb6pag/pdb
PDB entry 6pag coloured by chain, front view.
PDB entry 6pag coloured by chain, side view.
PDB entry 6pag coloured by chain, top view.
Source organisms:
Primary publication:
Structural plasticity of KIR2DL2 and KIR2DL3 enables altered docking geometries atop HLA-C.
Moradi S, Stankovic S, O'Connor GM, Pymm P, MacLachlan BJ, Faoro C, Retière C, Sullivan LC, Saunders PM, Widjaja J, Cox-Livingstone S, Rossjohn J, Brooks AG, Vivian JP
Nat Commun 12 2173 (2021)
PMID: 33846289

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-145271 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
HLA class I histocompatibility antigen, C alpha chain Chain: A
Molecule details ›
Chain: A
Length: 278 amino acids
Theoretical weight: 31.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P10321 (Residues: 25-302; Coverage: 81%)
Gene names: HLA-C, HLAC
Sequence domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Histone H3.1 Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 1.03 KDa
Source organism: synthetic construct
Expression system: Not provided
UniProt:
  • Canonical: P68431 (Residues: 41-49; Coverage: 7%)
Gene names: H3C1, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3FB, H3FC HIST1H3C, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J
Killer cell immunoglobulin-like receptor 2DL3 Chain: D
Molecule details ›
Chain: D
Length: 204 amino acids
Theoretical weight: 22.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P43628 (Residues: 22-225; Coverage: 64%)
Gene names: CD158B2, KIR2DL3, KIRCL23, NKAT2
Sequence domains: Immunoglobulin domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P64
Unit cell:
a: 111.855Å b: 111.855Å c: 87.994Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.224 0.223 0.254
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

5 review citations

Natural killer cells and immune-checkpoint inhibitor therapy: Current knowledge and new challenges.
Poggi et al. (2022)
4 more

1 mention without citation

Structural plasticity of KIR2DL2 and KIR2DL3 enables altered docking geometries atop HLA-C.
Moradi et al. (2021)