› 6pb0

Electron Microscopy
3Å resolution

Cryo-EM structure of Urocortin 1-bound Corticotropin-releasing factor 1 receptor in complex with Gs protein and Nb35

Released:
DOI: 10.2210/pdb6pb0/pdb
PDB entry 6pb0 coloured by chain, front view.
PDB entry 6pb0 coloured by chain, side view.
PDB entry 6pb0 coloured by chain, top view.
Source organisms:
Primary publication:
Molecular Basis for Hormone Recognition and Activation of Corticotropin-Releasing Factor Receptors.
Ma S, Shen Q, Zhao LH, Mao C, Zhou XE, Shen DD, de Waal PW, Bi P, Li C, Jiang Y, Wang MW, Sexton PM, Wootten D, Melcher K, Zhang Y, Xu HE
Mol Cell 77 669-680.e4 (2020)
PMID: 32004470
Related structures: EMD-20284

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-211088 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Corticotropin-releasing factor receptor 1 Chain: R
Molecule details ›
Chain: R
Length: 375 amino acids
Theoretical weight: 43.51 KDa
Source organism: Homo sapiens
Expression system: Spodoptera aff. frugiperda 2 RZ-2014
UniProt:
  • Canonical: P34998 (Residues: 24-427; Coverage: 89%)
Gene names: CRFR, CRFR1, CRHR, CRHR1
Sequence domains:
Urocortin Chain: U
Molecule details ›
Chain: U
Length: 40 amino acids
Theoretical weight: 4.7 KDa
Source organism: Homo sapiens
Expression system: synthetic construct
UniProt:
  • Canonical: P55089 (Residues: 83-122; Coverage: 40%)
Gene name: UCN
Sequence domains: Corticotropin-releasing factor family
Guanine nucleotide-binding protein G(i) subunit alpha-1; Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 378 amino acids
Theoretical weight: 43.9 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63096 (Residues: 1-3, 61-184; Coverage: 36%)
  • Canonical: P63092 (Residues: 4-67, 208-394; Coverage: 64%)
Gene names: GNAI1, GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 345 amino acids
Theoretical weight: 37.92 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Nanobody 35 Chain: N
Molecule details ›
Chain: N
Length: 140 amino acids
Theoretical weight: 15.34 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand CLR 5 x CLR
Ligand PLM 7 x PLM
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3Å
Relevant EMDB volumes: EMD-20284
Expression systems:
  • Spodoptera aff. frugiperda 2 RZ-2014
  • synthetic construct
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

6 review citations

Capturing Peptide-GPCR Interactions and Their Dynamics.
Kaiser et al. (2020)
5 more

7 mentions without citation

G protein-coupled receptors: structure- and function-based drug discovery.
Yang et al. (2021)
6 more