› 6pe0

Electron Microscopy
3.5Å resolution

Msp1 (E214Q)-substrate complex

Released:
DOI: 10.2210/pdb6pe0/pdb
PDB entry 6pe0 coloured by chain, front view.
PDB entry 6pe0 coloured by chain, side view.
PDB entry 6pe0 coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the AAA protein Msp1 reveals mechanism of mislocalized membrane protein extraction.
Wang L, Myasnikov A, Pan X, Walter P
Elife 9 (2020)
PMID: 31999255
Related structures: EMD-20320

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124022 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
AAA+ ATPase domain-containing protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 383 amino acids
Theoretical weight: 42.6 KDa
Source organism: Thermochaetoides thermophila
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G0S654 (Residues: 31-411; Coverage: 93%)
Gene name: CTHT_0034230
Sequence domains:
Unknown E. coli peptide Chain: G
Molecule details ›
Chain: G
Length: 10 amino acids
Theoretical weight: 869 Da
Source organism: Escherichia coli
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand ATP 5 x ATP
Ligand MG 5 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-20320
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli

Quick links

Citations

6 review citations

Quality control of the mitochondrial proteome.
Song et al. (2021)
5 more

3 mentions without citation

Structure of the AAA protein Msp1 reveals mechanism of mislocalized membrane protein extraction.
Wang et al. (2020)
2 more