› 6pe2

Electron Microscopy
4Å resolution

Drosophila P element transposase strand transfer complex

Released:
DOI: 10.2210/pdb6pe2/pdb
PDB entry 6pe2 coloured by chain, front view.
PDB entry 6pe2 coloured by chain, side view.
PDB entry 6pe2 coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Structure of a P element transposase-DNA complex reveals unusual DNA structures and GTP-DNA contacts.
Ghanim GE, Kellogg EH, Nogales E, Rio DC
Nat Struct Mol Biol 26 1013-1022 (2019)
PMID: 31659330
Related structures: EMD-20321

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120447 (preferred)
Entry contents:
2 distinct polypeptide molecules
3 distinct DNA molecules
Macromolecules (5 distinct):
Transposable element P transposase Chains: A, G
Molecule details ›
Chains: A, G
Length: 569 amino acids
Theoretical weight: 65.53 KDa
Source organism: Drosophila melanogaster
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q7M3K2 (Residues: 1-561; Coverage: 75%)
Gene name: T
Sequence domains:
Transposable element P transposase Chains: B, H
Molecule details ›
Chains: B, H
Length: 135 amino acids
Theoretical weight: 16.09 KDa
Source organism: Drosophila melanogaster
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q7M3K2 (Residues: 617-751; Coverage: 18%)
Gene name: T
Sequence domains: 87kDa Transposase
DNA (5'-D(P*CP*GP*AP*AP*CP*TP*AP*TP*A)-3') Chains: C, I
Molecule details ›
Chains: C, I
Length: 16 nucleotides
Theoretical weight: 4.83 KDa
DNA (27-MER) Chains: D, J
Molecule details ›
Chains: D, J
Length: 38 nucleotides
Theoretical weight: 11.7 KDa
DNA (56-MER) Chains: E, K
Molecule details ›
Chains: E, K
Length: 79 nucleotides
Theoretical weight: 24.4 KDa

Ligands and Environments

2 bound ligands:
Ligand GTP 2 x GTP
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4Å
Relevant EMDB volumes: EMD-20321
Expression system: Spodoptera frugiperda

Quick links

Citations

4 review citations

Structure and function of retroviral integrase.
Maertens et al. (2022)
3 more

2 mentions without citation

Structure of a P element transposase-DNA complex reveals unusual DNA structures and GTP-DNA contacts.
Ghanim et al. (2019)
1 more