› 6pe8

X-ray diffraction
2.84Å resolution

Crystal structure of CD40/ABBV-323 FAB complex

Released:
DOI: 10.2210/pdb6pe8/pdb
PDB entry 6pe8 coloured by chain, front view.
PDB entry 6pe8 coloured by chain, side view.
PDB entry 6pe8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
CD40/anti-CD40 antibody complexes which illustrate agonist and antagonist structural switches.
Argiriadi MA, Benatuil L, Dubrovska I, Egan DA, Gao L, Greischar A, Hardman J, Harlan J, Iyer RB, Judge RA, Lake M, Perron DC, Sadhukhan R, Sielaff B, Sousa S, Wang R, McRae BL
BMC Mol Cell Biol 20 29 (2019)
PMID: 31382872

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-231787 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
FAB Heavy chain Chains: A, H
Molecule details ›
Chains: A, H
Length: 223 amino acids
Theoretical weight: 23.75 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
FAB Light chain Chains: B, L
Molecule details ›
Chains: B, L
Length: 220 amino acids
Theoretical weight: 24.29 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Tumor necrosis factor receptor superfamily member 5 Chains: T, U
Molecule details ›
Chains: T, U
Length: 173 amino acids
Theoretical weight: 19.19 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P25942 (Residues: 21-193; Coverage: 67%)
Gene names: CD40, TNFRSF5
Sequence domains: TNFR/NGFR cysteine-rich region

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P21212
Unit cell:
a: 173.308Å b: 75.959Å c: 126.093Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.205 0.265
Expression systems:
  • Cricetulus griseus
  • Homo sapiens

Quick links

Citations

5 review citations

Recent advances in the development of protein-protein interactions modulators: mechanisms and clinical trials.
Lu et al. (2020)
4 more

1 mention without citation

CD40/anti-CD40 antibody complexes which illustrate agonist and antagonist structural switches.
Argiriadi et al. (2019)