› 6pek

Electron Microscopy
4.2Å resolution

Structure of Spastin Hexamer (Subunit A-E) in complex with substrate peptide

Released:
DOI: 10.2210/pdb6pek/pdb
PDB entry 6pek coloured by chain, front view.
PDB entry 6pek coloured by chain, side view.
PDB entry 6pek coloured by chain, top view.
Source organisms:
Primary publication:
Structure of spastin bound to a glutamate-rich peptide implies a hand-over-hand mechanism of substrate translocation.
Han H, Schubert HL, McCullough J, Monroe N, Purdy MD, Yeager M, Sundquist WI, Hill CP
J Biol Chem 295 435-443 (2020)
PMID: 31767681
Related structures: EMD-20327

Function and Biology Details

Reaction catalysed: 
n ATP + n H(2)O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-167238 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Spastin Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 498 amino acids
Theoretical weight: 54.5 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q9UBP0 (Residues: 87-616; Coverage: 81%)
Gene names: ADPSP, FSP2, KIAA1083, SPAST, SPG4
Sequence domains:
substrate peptide, TYR-GLU-TYR-GLU-TYR-GLU-TYR-GLU Chain: G
Molecule details ›
Chain: G
Length: 10 amino acids
Theoretical weight: 1.48 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand ADP 5 x ADP
Ligand BEF 4 x BEF
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.2Å
Relevant EMDB volumes: EMD-20327
Expression systems:
  • Saccharomyces cerevisiae
  • Not provided

Quick links

Citations

7 review citations

Cutting, Amplifying, and Aligning Microtubules with Severing Enzymes.
Kuo et al. (2021)
6 more

2 mentions without citation

Structure of spastin bound to a glutamate-rich peptide implies a hand-over-hand mechanism of substrate translocation.
Han et al. (2020)
1 more