› 6pfl

X-ray diffraction
2.1Å resolution

Crystal structure of Human HUWE1 WWE domain in complex with ADPR

Released:
DOI: 10.2210/pdb6pfl/pdb
PDB entry 6pfl coloured by chain, front view.
PDB entry 6pfl coloured by chain, side view.
PDB entry 6pfl coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Halabelian L, Zeng H, Dong A, Loppnau P, Bountra C, Edwards AM, Arrowsmith CH, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182304 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase HUWE1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 91 amino acids
Theoretical weight: 10.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7Z6Z7 (Residues: 1611-1700; Coverage: 2%)
Gene names: HSPC272, HUWE1, KIAA0312, KIAA1578, UREB1
Sequence domains: WWE domain

Ligands and Environments

2 bound ligands:
Ligand APR 2 x APR
Ligand UNX 3 x UNX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P3221
Unit cell:
a: 50.423Å b: 50.423Å c: 128.412Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.211 0.208 0.26
Expression system: Escherichia coli BL21(DE3)

Quick links