› 6pfn

X-ray diffraction
1.76Å resolution

Succinyl-CoA synthase from Francisella tularensis

Released:
DOI: 10.2210/pdb6pfn/pdb
PDB entry 6pfn coloured by chain, front view.
PDB entry 6pfn coloured by chain, side view.
PDB entry 6pfn coloured by chain, top view.
Source organism: Francisella tularensis subsp. tularensis SCHU S4
Entry authors: Osipiuk J, Maltseva N, Jedrzejczak R, Satchell KJF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
ATP + succinate + CoA = ADP + phosphate + succinyl-CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177704 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Succinate--CoA ligase [ADP-forming] subunit alpha Chains: A, C
Molecule details ›
Chains: A, C
Length: 296 amino acids
Theoretical weight: 30.95 KDa
Source organism: Francisella tularensis subsp. tularensis SCHU S4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5NHF4 (Residues: 1-290; Coverage: 100%)
Gene names: FTT_0503c, sucD
Sequence domains:
Succinate--CoA ligase [ADP-forming] subunit beta Chains: B, D
Molecule details ›
Chains: B, D
Length: 387 amino acids
Theoretical weight: 41.62 KDa
Source organism: Francisella tularensis subsp. tularensis SCHU S4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5NHF3 (Residues: 1-387; Coverage: 100%)
Gene names: FTT_0504c, sucC
Sequence domains:

Ligands and Environments


Cofactor: Ligand COA 2 x COA
2 bound ligands:
Ligand ACT 1 x ACT
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 69.073Å b: 158.735Å c: 73.017Å
α: 90° β: 113.11° γ: 90°
R-values:
R R work R free
0.187 0.186 0.218
Expression system: Escherichia coli BL21(DE3)

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