› 6pjv

X-ray diffraction
1.43Å resolution

Structure of Human Sonic Hedgehog in complex with Zinc and Magnesium

Released:
DOI: 10.2210/pdb6pjv/pdb
PDB entry 6pjv coloured by chain, front view.
PDB entry 6pjv coloured by chain, side view.
PDB entry 6pjv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of Sonic Hedgehog protein in complex with zinc(II) and magnesium(II) reveals ion-coordination plasticity relevant to peptide drug design.
Bonn-Breach R, Gu Y, Jenkins J, Fasan R, Wedekind J
Acta Crystallogr D Struct Biol 75 969-979 (2019)
PMID: 31692471

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172295 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sonic hedgehog protein N-product Chain: A
Molecule details ›
Chain: A
Length: 169 amino acids
Theoretical weight: 19.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15465 (Residues: 29-197; Coverage: 39%)
Gene name: SHH
Sequence domains: Hedgehog amino-terminal signalling domain

Ligands and Environments

5 bound ligands:
Ligand DMS 3 x DMS
Ligand GOL 1 x GOL
Ligand MG 2 x MG
Ligand ZN 1 x ZN
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: I23
Unit cell:
a: 118.728Å b: 118.728Å c: 118.728Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.124 0.124 0.139
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Hedgehog signaling in tissue homeostasis, cancers, and targeted therapies.
Jing et al. (2023)
1 other article

3 mentions without citation

Dispatched uses Na+ flux to power release of lipid-modified Hedgehog.
Wang et al. (2021)
2 more