› 6pkp

Electron crystallography
1.91Å resolution

MicroED structure of proteinase K from a platinum-coated, polished, single lamella at 1.91A resolution (#10)

Released:
DOI: 10.2210/pdb6pkp/pdb
PDB entry 6pkp coloured by chain, front view.
PDB entry 6pkp coloured by chain, side view.
PDB entry 6pkp coloured by chain, top view.
Source organism: Parengyodontium album
Primary publication:
Qualitative Analyses of Polishing and Precoating FIB Milled Crystals for MicroED.
Martynowycz MW, Zhao W, Hattne J, Jensen GJ, Gonen T
Structure 27 1594-1600.e2 (2019)
PMID: 31422911
Related structures: EMD-20362

Function and Biology Details

Reaction catalysed: 
Hydrolysis of keratin, and of other proteins with subtilisin-like specificity. Hydrolyzes peptide amides.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139275 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proteinase K Chain: A
Molecule details ›
Chain: A
Length: 279 amino acids
Theoretical weight: 28.93 KDa
Source organism: Parengyodontium album
UniProt:
  • Canonical: P06873 (Residues: 106-384; Coverage: 76%)
Gene name: PROK
Sequence domains: Subtilase family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 1.91Å
Relevant EMDB volumes: EMD-20362

Quick links

Citations

5 review citations

Electron Diffraction of 3D Molecular Crystals.
Saha et al. (2022)
4 more

1 mention without citation

Qualitative Analyses of Polishing and Precoating FIB Milled Crystals for MicroED.
Martynowycz et al. (2019)